Imaging and Staining
Immunofluorescence Staining
Visualisation of target proteins in cell and tissue samples with fluorescently labelled antibodies; high-resolution imaging of intracellular localisation.
About the service
Immunofluorescence staining makes specific proteins and biological molecules in cell and tissue samples visible through fluorescently labelled antibodies. The position of the target molecule inside the cell — nucleus, cytoplasm or membrane — can be observed directly.
The work covers fixation, permeabilisation, blocking, primary and secondary antibody incubations and nuclear counterstaining. Each protocol is optimised for the target antibody; dilution ratios and incubation times are agreed before the study.
Process
- Target protein and antibody suitability
- Fixation and permeabilisation
- Blocking and primary antibody
- Fluorescently labelled secondary antibody
- Nuclear staining and mounting
- Imaging and reporting
Deliverables
- High-resolution fluorescence microscope images
- Per-channel and merged image sets
- Publication-ready images with scale bars
- Detailed record of the protocol applied
- Signal intensity measurement on request
Sample and document requirements
Antibody details (catalogue number, host species, recommended dilution) and any previously tested protocol should be shared.